General Overview


CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology is a relatively facile method for editing genes. Editing allows researchers to elucidate the function of genes, leading to new products and
procedures with benefits to human health and well being. Gene-edited cells are a useful tool for understanding disease processes and how we might intervene.

The genes of these cells may be overexpressed, knocked-out, or otherwise
edited in various ways to mimic diseased cells that will respond to experimental treatment as would the tissues being modeled.

The Genome Editing Core Facility offers a suite of services to support researchers who use these kinds of cells:

  • Design and cloning of customized plasmids for lentiviral vector production
  • Lentiviral packaging and production for gene knockdown and overexpression
  • Customized cloning for CRISPR-based genome editing
  • On-site lentiviral transduction (BSL2 cell culture facility required)
  • Consultation on design of lentiviral vector and genome editing tools
  • Access to facility instrumentation and plasmid library

Equipment:

  • BSL2 Biosafety Cabinet
  • CO2 Incubator
  • Ultracentrifuge
  • Ultra-low freezer
  • Biomedical Refrigerator
  • Biomedical Freezer
  • Liquid Nitrogen Storage Tank
  • Multimode Plate Reader
  • Luminometer
  • Refrigerated Centrifuge
  • Vertical Polyacrylamide Gel Electrophoresis System
  • Real-Time PCR System
  • Inverted Fluorescence Microscope
  • Semi-Dry Transfer System

Serveices Offered by Genome Editing Facility

Guidelines on How to Avail the GEF Services

Downloadable Forms